Fig. 1. Ms/MsR1 regulation of crop enlargement.

a-c″, Crop phenotypes resulting from Ms-Gal4-driven Ms neuron activation/silencing. Ms-Gal4-driven TrpA1 activation enlarges the crop (a) compared to controls (a’,a″). Ms-Gal4-driven Kir2.1 silencing (temporally confined with tub-Gal80^TS) leads to smaller crops (b) compared to controls (b,b″). MsR1 downregulation in adult crop muscles (vm-Gal4-driven MsR1-RNAi expression, temporally confined with tub-Gal80^TS) leads to smaller crops (c), compared to controls (c’,c″). We note that vm-Gal4 is expressed in all visceral muscles, but leads to crop muscle-specific downregulation given the neuron- and crop muscle-specific MsR1 expression, Extended Data Fig. 5a-i’. d, Taotie-Gal4-driven, but not Mip-Gal4-driven Ms downregulation significantly reduces crop area (to a lesser degree than Ms neuron silencing, as expected from expression of Taotie-Gal4 in only a subset of PI Ms neurons, Extended Data 3p-p″). e, Myosuppressin receptor phylogeny. Scale bars: a-c″ = 500μm. In this and all subsequent figures, see Supplementary Information for a list of full genotypes, sample sizes and conditions. Statistics: Kruskal Wallis test. In this and all subsequent boxplots: line: median; box: 75^th-25th percentiles; whiskers: minimum and maximum. All data points are shown. *: 0.05>p>0.01; **: 0.01>p>0.001; ***: p<0.001.