Extended Data Fig. 7. Extended analysis of mast cells and IgE in human samples.

a, measurement of trypsin-like activity in supernatants of rectal biopsies from HV and IBS patients (n = 13 and 48, respectively) in basal conditions. b, c, number of (a) MC (CD117⁺) and (b) IgE⁺-MC (CD117⁺ IgE⁺) quantified in mucosal rectal biopsies from HV and IBS patients (n = 15 and 22, respectively). d, quantification of CD117⁺ IgE⁺ cells (MC) at ≤5 μm from nerve fibers (β tubulin III⁺ cells) in HV and IBS patients (n = 15 and 17, respectively). e, correlation between the IgE TCCF and max mucosal diameter after food-antigen injection in IBS patients (n = 7). f, number of IgE⁺ clones was quantified in mucosal rectal biopsies cDNA from HV and IBS patients (n = 10 and 20, respectively) using deep sequencing. Two-tailed Mann-Whitney test in a-d and f. two-tailed Pearson’s correlation in e. Data are shown as median ± IQR in a-d, and as box-and-whiskers (center line, median; box, 25^th and 75^th percentiles; whiskers, 10^th and 90^th percentiles) in f. HPF = high power field.