Fig. 3. A glr2.7/2.8/2.9 triple mutant is compromised in pattern-induced Ca²⁺ influx and bacterial disease resistance.

a, b, Parent (darker shades) or glr2.7/2.8/2.9 (lighter shades) YC3.6 reporter lines were assayed for response to a variety of patterns, salt (NaCl) or cold (4 °C) treatment; peak Ca²⁺ signal reported by YC3.6 within 25 min (patterns), 1 min (salt), or 5 min (cold) is shown. Each point represents peak ratio of YFP to CFP (proportional to Ca²⁺ concentration) for a single seedling, normalized to initial ratio.Different shapes represent 3-4 independent experiments, n=10-20 for each experiment/line/treatment combination. c, Parent and glr2.7/2.8/2.9 mutants in Col-0 and YC3.6 background were assayed for bacterial susceptibility, alongside the hypersusceptible bak1-5 mutant. Colony forming units (CFU) were counted two days post infiltration. Each point represents one infected plant and different shapes represent 3 independent experiments, n=5-7 for each experiment/line/treatment combination. Box plots center on the median, with box extending to the first and third quartile, and whiskers extending to the lesser value of the furthest point or 1.5x the inter-quartile range. Statistical tests were performed in R: ANOVA with experiment as a blocking factor, on square root of peak normalized Ca²⁺ response or log₁₀(CFU). Post-hoc tests were performed using the emmeans package in R: In a and b glr2.7/2.8/2.9 was compared to parent under each treatment, and in c (left), each genotype was compared to Col-0 (dunnettx method) and (right) YC3.6 glr2.7/2.8/2.9 was compared to YC3.6.