Extended Data Fig. 1. Quality control and exploratory analysis of RNA-seq data.

Expression changes in this study at a, 30 min and b, 3 h are plotted against previously published results for flg22, elf18/26, and chitooctaose (CO8). Linear correlation shown in red, with R² (linear regression) shown on each plot. c, PCA analysis of log₂(FC) of differentially expressed genes, showing (left) minimal changes in receptor-mutant treated plants, mostly corresponding with later time points, and rays of response (right) corresponding with plants at 30, 90, or 180 min post-treatment. d, Pearson correlation heatmap of DESeq2-calculated log₂(FC) showing clustering largely by time point, with the strongest correlations at 30 min.