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. 2021 Mar 10;13(2):025004. doi: 10.1088/1758-5090/abce0a

Figure 1.

Figure 1.

Microfabrication of polyacrylamide-co-acrylic acid (PAAm-co-PAAc) hydrogel and cell seeding. (A) Schematic representation of the microfabrication and cell-seeding workflow [1]: spin coating of photoresist (SU-8) [2], pattern transfer from photomask to photoresist by UV [3], pattern resulting from photolithography: arrays of pillars resembling the shape of adult CM [4], perfluorosilanization of substrate [5], PAAm-co-PAAc coating of the hard mold by drop-casting [6], removal of PAAm-co-PAAc and ECM conjugation [7], hiPSC-CMs seeding on the platform [8], hiPSC-CM culture on micropatterned PAAm-co-PAAc hydrogel. (B) Representative brightfield images of PAAm-co-PAAc hydrogels of (i) 70 × 14 µm, (ii) 40 × 8 µm, (iii) 98 × 14 µm and (iv) 56 × 8 µm. (C) Representative brightfield images of hiPSC-CMs cultured on (i) 70 × 14 µm, (ii) 40 × 8 µm, (iii) 98 × 14 µm and (iv) 56 × 8 µm. Scale bars: 25 µm.