(A) Schematic of the GAL locus showing the location of primer sets used for ChIP analysis.
(B) ChIP analysis of H3 K4 methylation and HA-Set1 binding. Cells were grown to log phase in rich media containing 2% galactose (GAL) or 2% glucose (GLU), and chromatin was precipitated with antibodies to H3 K4me2, H3 K4me3, and the HA-epitope. PCR was performed with primer sets shown in (A) and a telomeric primer set (TEL) as a loading control.
(C) Quantification of ChIP data as fold enrichment of signal at the GAL locus over telomeric signal normalized to the input.