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. Author manuscript; available in PMC: 2021 Jun 3.
Published in final edited form as: Mol Cell. 2008 Dec 5;32(5):685–95. doi: 10.1016/j.molcel.2008.09.027

Figure 1. H3 K4 Methylation Pattern at the GAL1–GAL10 Locus.

Figure 1

(A) Schematic of the GAL locus showing the location of primer sets used for ChIP analysis.

(B) ChIP analysis of H3 K4 methylation and HA-Set1 binding. Cells were grown to log phase in rich media containing 2% galactose (GAL) or 2% glucose (GLU), and chromatin was precipitated with antibodies to H3 K4me2, H3 K4me3, and the HA-epitope. PCR was performed with primer sets shown in (A) and a telomeric primer set (TEL) as a loading control.

(C) Quantification of ChIP data as fold enrichment of signal at the GAL locus over telomeric signal normalized to the input.