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. Author manuscript; available in PMC: 2021 Jun 4.
Published in final edited form as: Matrix Biol. 2019 Jul 8;85-86:15–33. doi: 10.1016/j.matbio.2019.06.009

Fig. 3.

Fig. 3

Methods for assaying cell growth and pluripotency in the 6 mg/mL peptide gels. (a) Real-time measurements of % increase in signal relative to day 0, from fluorescently-tagged cell lines encapsulated in matrix-free gels (seeding density 5 × 105 cells/mL). Graphs show mean ± standard deviation for n = 3 independent experiments. Trendlines are intended as a visual guide only. (b) End-point immunostaining and microscopy of unmodified mES (Oct4 stain) and MCF7 (CK18 stain) cell lines seeded within the matrix-free peptide gels. Scale bar 50 μm.