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. Author manuscript; available in PMC: 2021 Nov 30.
Published in final edited form as: Nat Methods. 2021 May 31;18(6):688–693. doi: 10.1038/s41592-021-01149-9

Extended Data Fig. 3. Effects of refractive index mismatch on the depletion and effective PSF.

Extended Data Fig. 3

The presence of refractive index mismatch between the silicone oil (ns) and the sample mounting medium (ni) causes the appearance of depth dependent side-lobes in the effective PSFs, even when correcting for piston. In addition, the main lobe of the effective PSF also shifts as a function of depth. All results are calculated for ns= 1.406 and ni = 1.33.