Extended Data Fig. 4. Relative fluorescence intensity of ten consecutive 3D data stacks.

(a) Sum projections of a representative series of ten 3D data stacks consecutively recorded of the same cell region (N=5 cell regions). A photobleaching curve is extracted for each series. (b) Photobleaching as a function of iterations. Data points and error bars represent the mean values of five different data sets and their standard deviations, respectively.