Table 1. Apparent K D determined by filter binding.
Protein | K D (nM) |
---|---|
RNase E (628–843) | 58±11 |
RhlB | 48±13 |
RhlB ΔCT | 193±54 |
RNase E (628–843)–RhlB | 25±6 |
RNase E (628–843)–RhlB ΔCT | 60±18 |
Dissociation constants were determined assuming simple one site binding. Each value is the average of at least two independent measurements. RhlB ΔCT lack the C-terminal basic region and corresponds to residues 1–397.