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. Author manuscript; available in PMC: 2021 Jun 16.
Published in final edited form as: J Mol Biol. 2007 Feb 7;368(3):639–51. doi: 10.1016/j.jmb.2007.01.080

Figure 4. Effects of Krebs metabolites on OGDH complex activity monitoring the NADH product of the reaction.

Figure 4

(a) Substrate-dependence of the rate of reaction of E. coli OGDH complex with E1o wild-type and mutants in which active site His residues (H298 and H260) are replaced with Ala. (b) The rate of the OGDH complex containing wild-type E1o or active site mutant of E1o. The assay was performed with 400 μM 2-oxoglutarate in the presence of varying concentrations of oxaloacetate.