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. Author manuscript; available in PMC: 2021 Aug 4.
Published in final edited form as: Nat Methods. 2021 Feb 4;18(2):186–193. doi: 10.1038/s41592-020-01054-7

Figure 6. Comparison of maps obtained from published tilt-series using M or other software.

Figure 6

(a) 80S ribosome data from EMPIAR-10064 were used to benchmark tilt-series processing in EMAN (EMD-0529). M achieved higher resolution, accompanied by visibly better resolved features such as RNA (green arrow) and α-helices (orange arrow).

(b) 80S ribosome data from EMPIAR-10045 were used to benchmark emClarity. The originally published map (EMD-8799, not shown) exhibited strong resolution anisotropy. A recently updated map42 still suffered from resolution anisotropy (“smearing” direction indicated by orange arrows). M achieved higher and more isotropic resolution, aiding the map’s interpretability.

(c) HIV-1 capsid-SP1 data from EMPIAR-10164 were used to benchmark emClarity (EMD-8986). M achieved slightly higher resolution using ca. 30% of the particle number used by emClarity. Doubling the number of particles did not increase the resolution. PDB-5L93 was rigid-body fitted into the maps for visualization.