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. Author manuscript; available in PMC: 2021 Jun 21.
Published in final edited form as: J Mol Biol. 2008 Jul 27;382(4):870–83. doi: 10.1016/j.jmb.2008.07.059

Fig. 3. Comparison of recombinant and cell-extracted degradosome.

Fig. 3

(a) SDS-PAGE analysis. Lane 1, the cell-extracted FLAG-tagged pull-down degradosome assembly grown at 37 °C; lane 2, recombinant assembly purified using high-salt/urea; lane 3, recombinant low-salt preparation. The change in the electrophoretic mobility of PNPase in lanes 2 and 3 is due to the presence of a hexahistidine tag. Molecular weight markers (in kDa) are indicated. (b) Native gel electrophoresis of degradosome samples. Lane 1, recombinant assembly; lane 2, FLAG-tagged degradosome. Bands were detected by immunoblotting with anti-RNase-E antibodies. After treatment with RNase A, the recombinant and cell-extracted degradosomes migrate at similar positions (right).