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. Author manuscript; available in PMC: 2021 Jun 22.
Published in final edited form as: Eur J Pharm Biopharm. 2019 Aug 21;143:61–69. doi: 10.1016/j.ejpb.2019.08.012

Fig. 6.

Fig. 6

Cellular uptake of redispersed NEM formulations in A549 cells. Median fluorescence intensity (MFI) was determined by flow cytometry to evaluate the cellular uptake in a human non-small cell lung carcinoma cell line (A549) of fresh or redispersed PEI polyplexes from 10% trehalose NEM formulations at N/ P ratios of 6, 8 and 10 with 0.5 μg of YOYO-1 labeled bulk DNA in comparison to freshly prepared formulations in presence of trehalose. Blank samples consisted of A549 cells treated with 5% glucose only. Data points indicate mean ± SD (n = 3). Two-way ANOVA, Bonferroni post-test, *P < 0.05, ns = non-significant.