(a) Protein extracts from A549 cells transfected with BSA nanoparticles loaded with 30 nM of scrambled control siRNA (siCtrl, Lane I), siRNA against KRAS GI2S (siKRAS GI2S, Lane 2) or siKRAS against both WT and mutant allele (siKRAS Total, Lane 3) were analysed by western blot using the indicated antibodies. Histone H3 (H3) was used as loading control. (b) Quantification of the KRAS knockdown observed in A. (c) Cell viablilty (% Y-axis) as measured by MTS assay in A549 cells transfected with siCtrl, siKRAS GI2S and siKRAS total. (d) Dot plot showing the percentage of Annexin V- or propidium iodide positive A549 cells after transfection with siCtrl, siKRAS GI2S or siKRAS Total. x and y axes denote Propidium iodide and Annexin V signals respectively, (e) Quantitation of Annexin V-positive cells represented as percentage of apoptotic cells. (f) A549 were subjected to transwell migration assays 24 h aftertransfection as indicated. The crystal violet dye staining images of the membranes are shown. (g) Percentage of migrated cells was quantified by counting three fields (20x magnification) per chamber and compared with controls. Magnification, X20X. Data points indicate mean ± SD, n = 3. One-way ANOVA, **, p < 0.01, ***, p < 0.001.