Table I. Differences in the three methods to prepare BSA nanoparticles.
2% BSA solution (ul) | Bulk DNA (ug) | EtOH volume 1 (ml) | 25% aqueous Glutaraldehyde (ul) | Ethanol volume 2 (ml) | |
---|---|---|---|---|---|
Method 1 | 500 | 150 | 1.0* | 4 | 1.0 |
Method 2 | 500 | 150 | 1.0 | 4 | 1.0 |
Method 3 | 500 | 150 | 1.4 | 4 | 0 |
In Method 1, BSA-DNA mixture was added dropwise over one minute into 1.0 ml EtOH