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. Author manuscript; available in PMC: 2021 Jun 21.
Published in final edited form as: Pharm Res. 2019 Jul 9;36(9):133. doi: 10.1007/s11095-019-2665-9

Table I. Differences in the three methods to prepare BSA nanoparticles.

2% BSA solution (ul) Bulk DNA (ug) EtOH volume 1 (ml) 25% aqueous Glutaraldehyde (ul) Ethanol volume 2 (ml)
Method 1 500 150 1.0* 4 1.0
Method 2 500 150 1.0 4 1.0
Method 3 500 150 1.4 4 0
*

In Method 1, BSA-DNA mixture was added dropwise over one minute into 1.0 ml EtOH