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. Author manuscript; available in PMC: 2021 Jun 24.
Published in final edited form as: Nat Med. 2020 Feb 10;26(2):193–199. doi: 10.1038/s41591-019-0734-6

Extended Data Fig. 10. Comparing MiXCR and 10X TCR data.

Extended Data Fig. 10

a-h, for 8 patients, indicated by number, CD8+ cell samples were subject to 10X chromium single cell 5’ RNA sequencing providing T cell receptor sequencing and standard bulk sequencing (see methods). Clones were identified by their β chain and for each productive β chain identified the relative clonal proportion (frequency) was calculated. Clones were matched via the CDR3 amino acid sequence with β chains from the same samples mapped from bulk CD8+ cell RNA using MiXCR. Where the clone fell below detection limit in MiXCR a value of 0 was attributed. MiXCR clones were identified for 92.1% clones >0.1% population in 10X (6990/7597) and 99.4% clones >0.2% size (5816/5852). x-axis= 10X proportion, y axis MiXCR proportion, r calculated using Pearson correlation coefficient, all P<2.2x10-16. i, Despite the different methods, the number of large clones (>0.5% total clonal population) identified from 10X and MiXCR approaches are correlated.