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. Author manuscript; available in PMC: 2021 Dec 1.
Published in final edited form as: Nature. 2021 Apr 7;594(7861):88–93. doi: 10.1038/s41586-021-03491-6

Extended Data Figure 7. Induction of Ca2+ oscillations by coronavirus Spike proteins and upon ACE2 and TMEM16F knockdown.

Extended Data Figure 7

a and c, Calcium transients over time in Vero cells co-transfected with GCaMP6s and the indicated plasmids and siRNAs. Data are expressed as ΔF/F over time (min); every line is one of at least twelve 180 μm2 ROI per condition, representing a group of 4 GCaMP positive cells on average.

b and d, Amplitude of calcium transient peaks per group of 4 GCaMP-positive cells in average in at least 12 180 μm2 ROI per condition. Data are expressed as ΔF/F; box: 25th-75th percentiles and median, whiskers: min-maxvalues; *P<0.05, **P<0.01, Kruskal-Wallis, two-sided, with Dunn’s correction for multiple comparisons.