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. Author manuscript; available in PMC: 2021 Jun 23.
Published in final edited form as: Circ Res. 2013 Apr 5;112(12):1583–91. doi: 10.1161/CIRCRESAHA.111.300119

Figure 4. Cezanne suppressed TRAF6 polyubiquitination in response to hypoxia-reoxygenation.

Figure 4

HUVEC were treated with Cezanne-specific siRNA (si-Cezanne from Dharmacon) or with a scrambled, non-targeting sequence and were then exposed to hypoxia (4 h) or hypoxia (4 h) followed by reoxygenation (2-20 h) or remained untreated. (A) TRAF6 immunoprecipitates or lysates were tested by Western blotting using anti-TRAF6 antibodies. (B) TRAF6 immunoprecipitates were tested by Western blotting using antibodies that recognise Lys63-polyubiquitin. n.s., non-specific. Data are representative of three independent experiments that gave closely similar results.