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. Author manuscript; available in PMC: 2021 Jun 23.
Published in final edited form as: Circ Res. 2013 Apr 5;112(12):1583–91. doi: 10.1161/CIRCRESAHA.111.300119

Figure 6. Cezanne reduced inflammation and injury in response to ischemia-reperfusion.

Figure 6

Left kidneys of wild-type or CezanneGT/GT mice (n=6 per group) were exposed to ischemia followed by reperfusion for 6 h whereas contralateral kidneys were uninjured. (A) Granulocytes were identified by napthol AS-D chloroacetate esterase staining. Representative images are shown and positive cells are indicated (arrow heads) (upper panels). Granulocytes were counted manually and data were pooled from multiple animals per group (lower panels). (B) Tissue sections were stained with hematoxylin and eosin prior to histological assessment. Representative images are shown with areas of acute tubular necrosis (arrows) and haemorrhage (arrow heads) indicated (upper panels). Quantitative assessment of tissue injury was carried out by two experienced renal researchers and average scores are shown (lower panels). 0, healthy; 1, mild injury; 2, moderate injury; 3, severe injury.