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GB1 constructs, spin label structures and simulated distance distributions. Top: Cartoon representations of GB1 constructs I6R1/K28R1 (left) and I6H/N8H/K28H/Q32H (right), with spin labels shown in stick representation. Bottom: Chemical structures of R1 nitroxide and double histidine CuII-NTA spin labels (left). Corresponding simulated distance distribution (right) for each construct, shown in black and red, respectively.
