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100 nM GB1 Q-band PELDOR data at 50K. Left: Background-corrected PELDOR data (black) and fit (grey) for 100 nM I6R1/K28R1 GB1. Right: Corresponding distance distribution given as 95% confidence intervals (± 2σ) with 50% noise added for error estimation during statistical analysis; simulated distance distributions are shown in red. Color bars represent reliability ranges (green: shape reliable; yellow: mean and width reliable; orange: mean reliable; red: no quantification possible).
