Figure 2. IRF5 deficiency protects against intestinal inflammation.

A) Representative H&E sections of colons from WT (left) and Irf5^-/- (right) mice at d21 Hh + αILI0R. B) Histopathology scoring of WT and Irf5^-/- colons. C) Number of cLPLs retrieved from steady state and d21 Hh + αILI0R WT and Irf5^-/- mice. D) frequencies of IFNγ-, IL17A- and IFNγ/IL17-producing CD4⁺ T cells in WT and Irf5^-/- mice following 4 hours of culture with PMA/Ionomycin and brefeldin assessed by intracellular flow cytometry. E) Spleen weights of WT and Irf5^-/- mice at steady state and d21 Hh + αILI0R. B,E: Data are representative of two independent experiments, (ss n=3, Hh + αILI0R n=7). Two-Way ANOVA with Tukey correction. C,D: Data are representative of two independent experiments, (WT ss n=6, Irf5^-/- ss n = 3 Hh + αIL10R n=7). Two-Way ANOVA with Tukey correction. F) The frequency of intestinal MNPs in the cLP at d21 Hh + αIL10R WT (n=12) and Irf5^-/- (n=11) mice. G) Representative H&E sections of colons from CX₃CR1^IRF5+ (left) and CX₃CR1^IRF5- (right) mice at d21 Hh + αIL10R. H) Histopathology scoring of colons from CX3CR1^IRF5+ (n=11) and CX3CR1^IRF5- (n=11) mice at d21 Hh + αIL10R. F,H: Data are pooled from two independent experiments. F) Two-Way ANOVA with Sidak correction, H) Unpaired t-test. Data presented are mean ± SEM, ns = not significant, * p ≤ 0.05, ** p ≤ 0.01, *** p ≤ 0.001, **** p < 0.0001