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. Author manuscript; available in PMC: 2021 Jun 25.
Published in final edited form as: Sci Immunol. 2020 May 22;5(47):eaax6085. doi: 10.1126/sciimmunol.aax6085

Figure 3. IRF5 has subtle effect on CD11c+ intestinal macrophages at steady state.

Figure 3

WT and Irf5-/- CD45+CD11b+SiglecF-Ly6G-CX3CR1+ cells were sorted from the colons of five mixed bone marrow chimera animals and subjected to scRNA-Seq analysis. A) Graph based clustering(58) of equal numbers of WT and Irf5-/- cells (n=4780 total) identified nine clusters of MNPs and one cluster of dendritic cells. B) The bar plots show the percentages of WT and Irf5-/- cells that were found in each cluster. Panels C) – F) show the expression of cell type markers C), genes expressed in Cd11c +ve macrophages D), genes expressed in Mrc1 +ve macrophages and genes with associated with macrophage differentiation and activation E).