Skip to main content
. Author manuscript; available in PMC: 2021 Jun 25.
Published in final edited form as: Sci Immunol. 2020 May 22;5(47):eaax6085. doi: 10.1126/sciimmunol.aax6085

Figure 4. IRF5 promotes generation of CD11c+ macrophages in inflamed colon.

Figure 4

WT and Irf5-/- CD45+CD11b+SiglecF-Ly6G-CX3CR1+ cells were sorted from the colons of three mixed bone marrow chimera animals at d21 of Hh + αIL10R colitis and subjected to droplet-based single cell transcriptomic analysis. A) Graph based clustering(58) of equal numbers of WT and Irf5-/- cells (n=1106 total) identified four clusters of MNPs and four clusters of dendritic cells. B) The violin plots show the expression levels (x axes) of selected known cLP MNP and DC sub-population markers in each of the identified clusters (y axes). C) The bar plots show the percentages of WT and Irf5-/- cells that were found in each cluster.