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. Author manuscript; available in PMC: 2021 Jul 7.
Published in final edited form as: J Am Chem Soc. 2020 Jul 28;142(32):13954–13965. doi: 10.1021/jacs.0c06541

Figure 4. SpCID1 efficiently uridylates poly(A) tail-containing sgRNA in the presence of UTP and AMUTP.

Figure 4

(a) Scheme showing uridylation reaction setup. (b) Uridylated sgRNA products were resolved by PAGE under denaturing conditions and were stained using Stains-All reagent.