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(a) Modified sgRNA-Cas9 complexes cleave target dsDNA. The target dsDNA in the presence of RNP complex produced two fragments of very similar length consistent with the site of cleavage. (b) Curve fits for the binding of unlabeled-(2), AMU-labeled (2Az), and Cy3 click-labeled (2 Cy3) sgRNAs to dCas9-eGFP derived from MST. Values are denoted as mean ± s.d. for n = 2 independent experiments.
