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. Author manuscript; available in PMC: 2021 Jul 7.
Published in final edited form as: J Am Chem Soc. 2020 Jul 28;142(32):13954–13965. doi: 10.1021/jacs.0c06541

Figure 6. Azide-labeled sgRNA forms a ternary complex with dCas9 and target DNA efficiently.

Figure 6

(a) Telomeric repeat-containing dsDNA target was incubated with dCas9 and sgRNA 1, UMP-tailed sgRNA 1UMP, azide-labeled sgRNA 1Az, biotin-conjugated sgRNA 1Bio, or Cy3-conjugated sgRNA Icy3. The formation of ternary complex (dCas9-sgRNA-dsDNA) was studied by PAGE under native conditions. (b) Fluorescence image of the gel at FAM wavelength. EMSA results suggest that posthybridization click functionalization is the most favorable approach to display functional tags on target gene.