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. Author manuscript; available in PMC: 2021 Jul 7.
Published in final edited form as: J Am Chem Soc. 2020 Jul 28;142(32):13954–13965. doi: 10.1021/jacs.0c06541

Table 1. Sequence of Model RNA Oligonucleotide and sgRNAs.

RNAa Sequence
5 ‘-FAM-labeled RNA 5’ FAM-UUACCAUAGAAUCAUGUGCCAUACAUCA 3’
sgRNA 1 5’GGGUUAGGGUUAGGGUUAGGGUUAGUUUAAGAGCUAUGCUGGAAACAGCAUAGCAAGUUUA AAUAAGGCUAGUCCGUUAUCAACUUGAAAAAGUGGCACCGAGUCGGUGCUUUAAAAAAAAAAAAAAA3’
sgRNA 1’ 5’GUUAGGGUUAGGGUUAGGGUUGUUUAAGAGCUAUGCUGGAAACAGCAUAGCAAGUUUAAAUAAGGCUAGUCCGUUAUCAACUUGAAAAAGUGGCACCGAGUCGGUGCUUUAAAAAAAAAAAAAAA 3’
sgRNA 2 5’GGCGAGGGCGAUGCCACCUAGUUUAAGAGCUAUGCUGGAAACAGCAUAGCAAGUUUAAAUAAGGCUAGUCCGUUAUCAACUUGAAAAAGUGGCACCGAGUCGGUGCUUUAAAAAAAAAAAAAAA 3’
a

Sequence of sgRNA targeting the protospacer region is represented in blue, and adenylate overhang is represented in red.