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. Author manuscript; available in PMC: 2021 Jul 9.
Published in final edited form as: Cancer Res. 2020 Jun 12;80(16):3399–3412. doi: 10.1158/0008-5472.CAN-19-3599

Figure 2. mIgG3 CH3 and to a lesser extent CH2 contribute to the direct cytotoxicity and improved avidity.

Figure 2

Constant domain shuffling suggests no significant contribution by CH1 to direct cytotoxicity (PI uptake, HCT15, A; proliferation inhibition on COLO205, B). In contrast, CH3 (1m3 and 3h3) contributes significantly to direct cytotoxicity, with a minor contribution by mCH2 only evident in a loss-of-function approach (3h2) (PI uptake, HCT15, C; proliferation inhibition, COLO205, D). Significance versus the respective parental constructs was deduced from two-way ANOVA. Significantly increased avidity and decreased off-rate by 1m3; with significantly decreased avidity and increased off-rate by 3h2 and more pronounced by 3h3, confirming the major CH3 and minor CH2 contributions (E, F). Significance deduced using one-way ANOVA, with Dunnett’s corrections for multiple comparisons.