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. Author manuscript; available in PMC: 2021 Jul 12.
Published in final edited form as: J Cell Sci. 2020 Dec 24;133(24):jcs246819. doi: 10.1242/jcs.246819

Fig. 7. Knocking down VPS35 does not affect cell viability but lowers intracellular copper content.

Fig. 7

(A) Graphical representation of cell viability where VPS35 expression was silenced by lentiviral mediated delivery of shRNA against VPS35 or not (non-targeting shRNA) as measured by Neutral Red (NR) assay. The assay was performed in two different copper conditions (basal and 50μM). Each conditions has four replicates. (B) Intracellular copper content (ng/g) as measured by ICP-OES in cells subjected to identical knockdown or copper treatment conditions as of 7A. Each conditions has five replicates (P<0.05). Graphs show mean±s.d. **P<0.01; ns, not significant (non-parametric Mann–Whitney U test).