Table 1. Mutagenic primer pairs used to generate the various mutations in the recombinant RNA helicases.

In lower case and underlined are the nucleotides that are changed from the original sequence to generate the respective helicase mutation.

Mutation	Mutagenic primer pairs
RhlB
F10A	5’-CAGAACAGAAGgçTTCCGACTTCGCCC-3’
	5’ -GGGCGAAGTCGGAAgçCTTCTGTTCTG-3’
F10M	5’-CAGAACAGAAGaTgTCCGACTTCGCCC-3’
	5’ -GGGCGAAGTCGGAcAtCTTCTGTTCTG-3’
H320D	5’-GCCGCGCGTGGTTTGUgATATTCCGGCAGTGACG-3’
	5’ -GCGTCACTGCCGGAATATcCAAACCACGCGCGG-3 ‘
S381A	5’-GGTCACTCAATTCCGGTAgçCAAATACAATCCGGACG-3’
	5’-CGTCCGGATTGTATTTGgçTACCGGAATTGAGTGACC-3’
Y383A	5’-CCGGTAAGCAAAgçCAATCCGGACGCATTG-3’
	5’-CAATGCGTCCGGATTGgçTTTGCTTACCGG-3’
RhlE
D310H	5’-CGCTGCGCGCGGCCTGçATATTGAAGAGCTGC-3’
	5’ -GCAGCTCTTGAATATgCAGGCCGCGCGCAGCG-3’
SrmB
D313H	5’ -GCCGCGCGCGGTATCçACATTCCTGACGTCAG-3’
	5’-CTGACGTCAGGAATGTgGATACCGCGCGCGGC-3’