Table 4. ATPase activity of the co-expressed Phe-10 variants of RhlB in complex with RNase E-(696–762).
Protein | Activity without RNAa | Activity with RNAa |
---|---|---|
F10A/RNase E-(696–762) | 1.1 (0.6) | NDb |
F10M/RNase E-(696–762) | 5.4(0.7) | ND |
a The activities are expressed in moles of inorganic phosphate released per minute per mol of protein (mol/Pi/min/mol protein). The errors given in parentheses are the mean ± S.D.
b Activity not detectable after 10 min.