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. Author manuscript; available in PMC: 2021 Jul 15.
Published in final edited form as: Free Radic Biol Med. 2020 Feb 1;152:797–809. doi: 10.1016/j.freeradbiomed.2020.01.177

Figure 6. Specific PMP-4 expression in the hypodermis is essential to maintain axonal integrity and locomotion.

Figure 6

(A) Locomotion behaviour expressed as thrashes per minute at L4+7 days in juIs76 [Punc-25::gfp], pmp-4(ok396); juIs76 [Punc-25::gfp] and pmp-4(ok396; juIs76 [Punc-25::gfp]) animals where PMP-4::mCherry is expressed in hypodermis (pmp-4(ok396); juIs76 [Punc-25::gfp]; Ex177 [Pdpy-7::pmp-4::mCherry]), in intestine (pmp-4(ok396); juIs76 [Punc-25::gfp]; Ex151 [Pges-1::pmp-4::mCherry]) or in neurons (pmp-4(ok396); juIs76 [Punc-25::gfp]; Ex191 [Punc-25::pmp-4::mCherry]) (n= 19 to 23 animals by condition). Representative confocal pictures showing axonal damage and quantitative analysis in (B) juIs76 [Punc-25::gfp] (n=22), (C) pmp-4(ok396); juIs76 [Punc-25::gfp] animals (n=19), pmp-4(ok396) animals where PMP-4::mCherry is expressed in (D) hypodermis (pmp-4(ok396); juIs76 [Punc-25::gfp]; Ex177 [Pdpy-7::pmp-4::mCherry]) (n=23), (E) intestine (pmp-4(ok396); juIs76 [Punc-25::gfp]; Ex151 [Pges-l::pmp-4::mCherry]) (n=22) or (F) neurons (pmp-4(ok396); juIs76 [Punc-25::gfp]; Ex191 [Punc-25::pmp-4::mCherry]) (n=19). The red colour in all pictures corresponds to the strong expression in the pharynx of the co-injection marker Pmyo-2::mCherry. White asterisks label the axonal abnormalities. Worms are oriented head to the left and dorsal face-up. Scale = 25 μm. Data represent the mean ± standard deviation (SD). Statistical analysis was carried out with two-way ANOVA, followed by Tukey’s post hoc test (*P<0.05; **P<0.01; ***P<0.001).