Figure 1. Male meiocytes exhibit high CG/CHG and low CHH methylation.
a, Model of male sexual lineage development in Arabidopsis thaliana. n, the number of chromosomes in the haploid genome. b, Heat maps showing CG, CHG and CHH methylation of the male sexual lineage comprising the meiocyte (Me), microspore (Mi), sperm (Sp) and vegetative cell (Vg), in comparison to rosette leaf (Rs). Methylation is calculated and presented in 10 kb windows, with the maximum set at the highest value among the five tissues for each context. The region enriched with mitochondria sequences on Chromosome 2 (3.23 to 3.51 Mb) is removed. c, Box plots demonstrating CG methylation for individual CG sites located within annotated transposons, and with methylation greater than 50% and at least 10 informative sequenced cytosines. Each box encloses the middle 50% of the distribution, with the horizontal line making the median, and vertical lines marking the minimum and maximum values that fall within 1.5 times the height of the box.