a, Box plots showing the absolute methylation at SLHs in the drm1drm2 (drm) and rdr2 mutants in comparison to wild type. b, Box plots demonstrating the abundance of 24 nucleotide (nt) small RNA in pollen or shoot at SLHs and non-SLH RdDM target loci. *P < 0.001; Kolmogorov-Smirnov test. c, Box plots showing the absolute methylation at SLH and non-SLH 50 bp windows in ros1dml2dml3 (rdd) mutant rosette leaf, wild-type rosette leaf, and wild-type male sex cells (meiocyte, microspore and sperm).