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. Author manuscript; available in PMC: 2021 Jul 19.
Published in final edited form as: Nature. 2019 Mar 20;567(7749):545–549. doi: 10.1038/s41586-019-1030-9

Figure 3. BRCA1 is required for MYCN-dependent elongation by RNAPII.

Figure 3

a: Metagene plots (mean+SEM as shadow) of total RNAPII after 4-OHT treatment (3 h) in control and BRCA1-depleted cells with shBRCA1#2 for 914 MYCN-activated genes (n=2).

b: Correlation of gene expression (n=3) with RNAPII occupancy at promoters in control and BRCA1-depleted cells with shBRCA1#2. Genes were grouped into 30 bins (300 genes/bin) and the mean for each bin is plotted with 95 % confidence interval.

c: Strand-specific DRIPc-seq data stratified by position of MYCN binding site.

d: DRIP documenting R-loops at the indicated loci upon depletion of BRCA1 and activation of MYCN (4 h). Data are normalized to control (shSCR, EtOH). Shown is the mean of technical triplicates with SD (n=4).

e: Density plot (mean+SEM as shadow) of DCP1A occupancy after 4-OHT treatment (5 h) in control or BRCA1-depleted cells with shBRCA1#2.

f: Genome browser tracks of the TFAP4 locus show chromatin association of DCP1A in cells treated as above.