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. Author manuscript; available in PMC: 2021 Jul 19.
Published in final edited form as: Free Radic Biol Med. 2020 May 6;154:105–118. doi: 10.1016/j.freeradbiomed.2020.04.029

Figure 5. Two rounds of screening identified 17 ORFΔcoq2Δ double mutants to have a diminished response to exogenous CoQ6.

Figure 5

(A) First, the degree of rescue of the ORFΔcoq2Δ candidates was calculated in comparison with the positive control: coq2Δ + CoQ6 . Of a total of 40 candidates, 23 showed a significantly decreased ability to rescue in presence of exogenous CoQ6. (B) Since a single deletion can compromise the ability of a mutant to grow on YPG, the degree of rescue of the previous significant candidates was calculated in comparison to the corresponding ORFΔ in the second phase of the screening. The emilΔcoq2Δ and qcr9Δcoq2Δ mutants were eliminated for further analysis due to special phenotypes (see main text). 17 candidates display impaired rescue in response to treatment with exogenous CoQ6. In both cases, columns represent the average degree of rescue (in %) ± SD of mutants growing on YPG in presence of 2 μM CoQ6 for 7 days. Three or more replicas were included for all candidates. The normalization variable is considered 100% and represented as a dashed line. Asterisks denote the different degrees of significance: *p<0.05, **p<0.01, ***p<0.001, and ****p<0.0001.