Table 1. Screening results for the 51 Hits.

1. Percentage of cell death obtained in the primary screening as in Figure 1C. 2-3. LC₅₀ ± 95%CI calculated with Graph Pad Prism 5.0 from percentages of cell dead at 8 different concentrations. An LC₅₀ >60 means that the compound didn’t showed activity at the measured concertation range. 4. Correspond to the concertation that showed higher spot area with minimum cell death. 5. Average “Spot_Total_Area_PerObject” ± SD, triplicate samples. Asterisk indicate statistical difference with respect to Staurosporine (negative control), with p-values expressed as: *** <0.001, ** 0.001-0.01, * 0.01-0.05 (2 tailed, equal variance t-Test). Values are represented in Figure 5A. (#) Compounds that showed no activity in primary screening but were selected to follow-up due to its structural similarities with the hits. 7. The positive and the negative control were present in all the screening plates in 5 replicates. The value showed here is the average ± SD between all screening plates. 8. Siramesine concertation curve was included in all the screening plates to access variability between the plates. The value showed here is the average ± SD of 9 curves across the screening plates. LT = Lysosomotropic, LCD = Induce Lysosomal cell death, ASM = Induce inhibition of Acid Sphingomyelinase, CAD = Cationic Amphiphilic Drug.