Figure 3. Oligonucleotides inhibit telomerase activity in a sequence-dependent manner.

(A) Telomerase activity was assayed in MDA-MB-435, MDA-MB-231/H2N, SKBR3 and U2OS cell lysate following treatment with 300 nM Match or Scramble oligonucleotide. Data were compared using one-way ANOVA with post hoc Tukey test. (B) Telomerase activity of MDA-MB-435 cell lysate after treatment with increasing concentration of Match, Mismatch 1, Mismatch 2, and Scramble oligonucleotides. (C) Telomerase activity of MDA-MB-435 cell lysate after treatment with increasing concentration of Match and Scramble oligonucleotides following DTPA-conjugation. (D) Telomerase activity of MDA-MB-435 cell lysate after pre-incubation with ¹¹¹In-DTPA-Match and ¹¹¹In-DTPA-Scramble (27 MBq/nmol) in the modified TRAP assay. Data were fitted using fixed-sloped non-linear regression and compared by F-test. ** P < 0.01, * P < 0.05. n=2, data represents three independent repeat experiments.