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. Author manuscript; available in PMC: 2021 Jul 20.
Published in final edited form as: Cancer Res. 2019 Jul 16;79(18):4627–4637. doi: 10.1158/0008-5472.CAN-18-3594

Figure 4. Cellular uptake and subcellular distribution of hTR-targeting oligonucleotides.

Figure 4

(A-D) 250 μL of 111In-oligonucleotides (220 nM, 3.6 MBq/nmol) or 111InCl3 were combined with transfection reagent and incubated with (A) MDA-MB-435, (B) U2OS, (C) SKBR3 and (D) MDA-MB-231/H2N cells for the indicated time. Cells were washed and lysed, and the proportion of internalised radioactivity determined by gamma-counting. n=6, data from three independent repeat experiments. (E) Subcellular localisation of fluorophore-labeled oligonucleotide inhibitors of telomerase in MDA-MB-435 cells. Cy3-labeled Match and Scramble oligonucleotides (220 nM) were transfected into MDA-MB-435 cells for 2.5 or 24 hours before fixation. Cells were counterstained for the Cajal body protein coilin, and DNA (DAPI). Images represent data from three independent repeat experiments. Scale bar 40 μm.