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. Author manuscript; available in PMC: 2022 Feb 18.
Published in final edited form as: Mol Cell. 2021 Jan 15;81(4):830–844.e13. doi: 10.1016/j.molcel.2020.12.035

Figure 1. Binding of PAF1c to MYC enhances association of MYC with active promoters.

Figure 1

A. Diagram of MYC protein structure showing the position of MYCBoxes and the GST-MYC construct used for pulldown experiments.

B. Coomassie gel showing purified proteins. 10% of input material is shown.

C. Immunoblots of MYC and PAF1c (n=3; in all legends, n indicates the number of independent biological replicates).

D. Immunoblots of pulldown experiment using GST-△MYCBox I and GST-△MYCBox II constructs (n=3).

E. Immunoblot showing levels of CTR9, CDC73 and MYC in U2OS cells after stable expression of constitutive shRNAs. Vinculin (VCL) was used as loading control (n=3).

F. Density plot of MYC centered on the transcription start site (TSS) of 8,437 active promoters in a ChIP-Rx experiment in control U2OS cells or in cells expressing shCTR9 or shCDC73 (n=1). All ChIP-Seq traces show S.E.M. as a shade.

G. Density plot of MYC as in (F) in a ChIP experiment in control U2OS cells or in cells stably expressing MYC and Dox inducible shRNA targeting CTR9 (shCTR9) or shCDC73. (n=1). See also Figure S1.