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. Author manuscript; available in PMC: 2021 Jul 20.
Published in final edited form as: Cancer Res. 2020 Nov 6;81(1):103–113. doi: 10.1158/0008-5472.CAN-20-1764

Figure 4. HOTAIR-sbid interferes with the SNAIL/HOTAIR/EZH2-mediated promoters H3K27me3.

Figure 4

qPCR analysis of ChIP assays with anti-H3K27me3 antibody (H3K27me3) and, as control, normal rabbit IgG (IgG) on chromatin from BW1J cells (a) and Hep3B (b) expressing HOTAIR-sbid (sbid) or the empty vector as a control (ctr). Data show the enrichment of H3K27 trimethylation on the Snail consensus binding sites on the murine promoters of HNF1α, HNF4α and E-cadherin. Rpl30 promoter was used as a negative control. Values derived from five independent experiments are reported as means ± s.e.m. and expressed as the percentage of the Input chromatin (% Input). Statistically significant differences are reported (*P<0.05).