| Antibodies |
| Rat monoclonal anti-E-cadherin |
DSHB |
Cat#DCAD2, RRID:AB_528120 |
| Mouse monoclonal anti-β-catenin |
DSHB |
Cat#N27A1, RRID:AB_528089 |
| Rat monoclonal anti-α-catenin |
DSHB |
Cat#DCAT-1, RRID:AB_532377 |
| Alexa Fluor 568 Phalloidin |
Thermo Fisher Scientific |
Cat#A12380 |
| Rabbit monoclonal anti-zipper |
Lecuit Laboratory |
16
|
| Chemicals, peptides, and recombinant proteins |
| Ethyleneglycol-bis(2-aminoethylether)-N,N,N′,N′-tetraacetic acid (EGTA) |
Sigma Aldrich |
Cat#E0396 |
| ROCK Inhibitor, H 1152 dihydrochloride |
Tocris |
Cat#2414 |
| Actin inhibitor, Lat A |
Thermo Fisher Scientific |
Cat#L12370
|
| Sodium Dithionite |
Sigma Aldrich |
Cat#157953 |
| Experimental models: organisms/strains |
|
D.melanogaster: endo dE-cad::GFP,sqh Sqh::mCherry |
Sqh-mCherry gift from Adam C Martin |
Recombinant used in51
|
|
D.melanogaster: endo dE-cadΔP::GFP/Cy |
This manuscript, Lecuit Laboratory |
28
|
|
D.melanogaster: Ubi-VSV-G::GFP |
Lecuit Laboratory |
44
|
|
D.melanogaster: UAS-dE-cad::GFP |
Gift from H.Oda |
52
|
|
D.melanogaster: Col-GAL4 |
Gift from C. Dearolf |
53
|
|
D.melanogaster: UAS-dE-cad-Δβcat::GFP |
This manuscript, Lecuit laboratory |
34
|
|
D.melanogaster: UAS-dE-cad-Δβcat-αcatABD::GFP |
This manuscript, Lecuit laboratory |
34
|
|
D.melanogaster: UAS-dE-cad::YFP |
This manuscript, Lecuit laboratory |
52
|
|
D.melanogaster: UAS-dE-cad-Δβcat::YFP |
This manuscript, Lecuit laboratory |
34
|
|
D.melanogaster: w1118; P{GD1695}v7916 |
Vienna Drosophila Resource Center |
VDRC:v7916 |
|
D.melanogaster: w1118; P{GD1566}v7819 |
Vienna Drosophila Resource Center |
VDRC:v7819 |
|
D.melanogaster: UAS-dEcad-ΔP |
Gift from H.Oda |
28
|
|
D.melanogaster: Hml-GAL4,UAS-2xEGFP |
Bloomington Drosophila Stock Center |
BL-30140 |
| Software and algorithms |
| MATLAB, Image processing toolbox |
Mathworks Inc |
https://www.mathworks.com/products/matlab.html
|
| ImageJ |
N/A |
https://imagej.nih.gov/ij/index.html
|
| Metamorph 7.0 software |
Molecular Devices Corporation, CA, USA |
https://www.moleculardevices.com/products/cellular-imaging-systems/acquisition-and-analysis-software/metamorph-microscopy
|
| GraphPad Prism |
GraphPad |
https://www.graphpad.com/scientifc-software/prism/
|
| OriginPro 8 |
Origin Lab |
https://www.originlab.com/
|
