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. Author manuscript; available in PMC: 2021 Jul 21.
Published in final edited form as: Nat Plants. 2020 Jun 29;6(7):766–772. doi: 10.1038/s41477-020-0695-2

Extended Data Figure 8. Cas9p-mediated genome editing in proximal stem cells induces cell death.

Extended Data Figure 8

(a) Stem cell death surrounding the QC was observed after one-day induction of ip35S>>Cas9p-PLT2. Based on cell types, the cell death response is classified into three categories: provascular cell death, LRC/epidermis initial cell death and columella initial cell death. Samples were counted twice if they had cell death in two different categories. (b) Cell death of provascular cells and early descendants was induced after one-day induction of ipWOL>>Cas9p-tagRFP-PLT2/RBR/GNOM. Cell walls are highlighted by propidium iodide (PI). Under PI detection settings, Cas9p-tagRFP is also visible. Numbers indicate the frequency of the observed phenotype in independent T1 samples analyzed. Experiments were repeated three times. Scale bars, 50 μm.