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. Author manuscript; available in PMC: 2021 Jul 21.
Published in final edited form as: Cancer Discov. 2020 Dec 16;11(5):1228–1247. doi: 10.1158/2159-8290.CD-20-0652

Figure 2. Translation and proliferation are uncoupled following Kras mutation.

Figure 2

(A) Growth of Apcfl/fl or Apcfl/fl KrasG12D/+ organoids, following 30hour treatment with 1μM rapamycin, relative to vehicle-treated organoids. Technical triplicates were performed and averaged to show the biological replicates as plotted. P values are from paired student t tests. The relative growth for untreated Apcfl/fl and Apcfl/fl KrasG12D/+organoids is set as 0. (B) Protein synthesis in small intestinal organoids of the indicated genotypes following 6hours of treatment with 250nM rapamycin or vehicle. Values are from 3 independent biological replicates ±SEM, represented relative to Apcfl/fl vehicle treated organoids. P values are from paired student t tests or a Mann Whitney test. See also Figure S2.