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. Author manuscript; available in PMC: 2022 Jan 5.
Published in final edited form as: Nat Plants. 2021 Jul 5;7(7):923–931. doi: 10.1038/s41477-021-00950-w

Extended Data Fig. 4. Control reactions for VC1 and vc1 primer pairs.

Extended Data Fig. 4

1 ng of pGEM-T plasmids carrying VC1 cloned from V. faba cv. Hedin/2 (Hed) or vc1 from Mélodie/2 (Mel) were subjected to PCR with primer pairs for either VC1 or vc1. The reaction mixtures and temperature program are the same as described in Methods for “specific amplification of VC1 and vc1 from seed coat cDNA of Hedin/2 and Mélodie/2”, except 25 cycles were used for the vc1 primer pair and 35 for the VC1 primer pair to account for their different efficiencies. This control experiment was carried out twice with similar results.