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A. Effect of interrupting His tag sequences with other amino acids (X): HHHHHH (purple), HHHH (yellow), HXHXHX (red), HXHX (green), and HXXH (blue). B. relationship between pI and radiochemical yield plotted separately for sequences with HHHHHH (purple), HXHXHX (red), HXHX (green), and HXXH (blue). Lines correspond to a monoexponential fit for each group.
