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. Author manuscript; available in PMC: 2021 Jul 22.
Published in final edited form as: Bioconjug Chem. 2020 Nov 26;32(7):1242–1254. doi: 10.1021/acs.bioconjchem.0c00561

Figure 5. Effect of positively and negatively charged amino acids on [“mTc][Tc(CO)3]+ labeling efficiency.

Figure 5

A. Comparison of the radiolabeling of all peptide sequences with at least 2 negatively charged amino acids (Glu and/or Asp, mean pl 11.1) and at least 2 positively charged amino acids (Arg and/or Lys, mean pI 5.4). The black lines represent the average radiochemical yield for each group. B. Effect on labeling efficiency of paired “point mutation” replacing Glu (E) with Arg (R) in the seven otherwise identical His tag variants where such comparison was possible. ***p < 0.001; **p < 0.01.