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. Author manuscript; available in PMC: 2021 Jul 22.
Published in final edited form as: Bioconjug Chem. 2020 Nov 26;32(7):1242–1254. doi: 10.1021/acs.bioconjchem.0c00561

Figure 9. Labeling efficiency of five His-tagged proteins with [99mTc(CO)3]+ in PBS during 120 min at 37 °C, at protein concentrations from 1.8 to 14.1 μM.

Figure 9

Only J591scFvJWT (blue line) contains an optimized His-tag. There are no data for the highest and the highest two concentrations, respectively, of 6C7.1scFv and 6C7.1scFvCys because restricted quantities were available. Data for the extended concentration range (0.9–28 μM) are shown in SI Figure S32.